THE INCORPORATION OF C14-GLUCOSE AND C14-RIBOSE INTO MOUSE LIVER DIPHOSPHOPYRIDINE NUCLEOTIDE

Incorporation of C14-labeled substrates into violacein.

Previous studies on the physiological aspects of violacein biosynthesis (DeMoss and Evans, 1959) suggested that only L-tryptophan would serve as the primary source of carbon for pigment formation by Chromobacterium violaceum. In view of the pigment structure suggested by Ballantine et al. (1958), 5-[3-(5-hydroxyindolyl)]-3-(3-isatinyl)-2-pyrrolone, it seemed advisable to determine the actual so...

The incorporation of formate-C14, glycine-2-C14, adenine-4, 6-C14, and phosphate-P32 into nucleic acids.

nine labeled either with C'4 or N'5. Considerable controversy has developed as to which one of these tracer compounds gives an accurate measurement of the “relativeturnover-rate― of the nucleic acids. In order to determine the “absolute turnover rate― of the nucleic acids, it is necessary to know the specific activity of the immediate precursor of the nucleic acids. Since even the ident...

Energy-linked Incorporation of Diphosphopyridine Nucleotide into Rat-liver Mitochondria.

The incorporation of formate-C14 into citrovorum factor.

Jukes (1) and Broquist et al. (2) reported that the addition of formate or serine to incubating cells of an A-Methopterin-resistant strain of Xtreptococcus.faecalis increased the amount of citrovorum factor (CF, folinic acid) which was formed from pteroylglutamic acid (PGA). This finding suggested that ‘Lformate-carbon” was incorporated into the structure of CF. Under appropriate conditions an ...

The incorporation of glycine-2-C14 into acid soluble nucleotide purines.

Acid-soluble nucleotides have recently been strongly implicated as precursors of polynucleo tides. Several workers using glycine-2-C'4 (7, 16) and adenine-C'4 (2, 10, 19, 21) have reported the rapid turnover of acid-soluble adenine with respect to nucleic acid adenine in a variety of biological systems. In experiments carried out for extended time periods after the administration of orotic acid...